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Main characters of the series
The series was created and is drawn by Gosho Aoyama. The protagonists from his earlier series Magic Kaito look very similar: Kaitou Kid has made a number of appearances as himself in the anime and manga of the series. The main protagonist of the Detective Conan series, Shinichi Kudo, and the protagonist of the Magic Kaito series, Kaito Kuroba, are also similar. Hundreds of minor characters have been created and are always given backgrounds, motives, and personalities that put them under suspicion during the investigation.
The English adaption dubbed by Funimation, called Case Closed, has different names than the one in the manga published by Viz Media.
Main character of the series and Ran's love interest. He was shrunk into a child after being forced to take a poison, called APTX 4869, created by the Black Organization.
"Child" version of Shinichi Kudo. He's after the Black Organization to regain his original body. The show follows his journey and the different cases he encounters along the way.
Shinichi's childhood friend, and main love interest. She doesn't know Conan's real identity. She's the one taking care of him along with her detective father, Kogoro. She is also skilled in karate.
Private eye and Ran's father. Separated from Eri Kisaki, Ran's mother.
Detective Boys
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| style="width:60px;"| || style="width: 125px; line-height: 15px;" align="center"|Ai Haibara || style="width: 125px; line-height: 15px;" align="center"|Vi Graythorn (movies) Anita Hailey (manga) || style="width: 600px; padding: 0 5px;"|Member of the Detective Boys and an adult shrunken by APTX 4869. Her former identity was a Black Organization scientist codenamed "Sherry", real name "Shiho Miyano". She invented APTX 4869.
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The Detective Boys are called the "Junior Detective League" in the English version of the show. Notably, the Detective Boys are the only people that see the bowtie in action who aren't suspicious about Conan's identity. They have seen Conan use the bowtie, and even caught Conan using the bowtie to solve cases.
Member of the Detective Boys. Has a crush on Ayumi and Haibara. The most intelligent true child in the Detective Boys. He is great when it comes to science and logic.
Ran's mother, a very successful attorney. Married to, but currently living separately from Kogoro Mouri. She is a friend of Yukiko Kudo from high school.
Shinichi's mother. A retired actress, she can disguise well and can mimic voices without a voice changer, a skill she learned from the deceased magician Toichi Kuroba. She is a close friend of Sharon Vineyard.
Shinichi's rival detective and best friend from Osaka. Known as "Great Detective of the West". He's also a trained Kendo martial artist and the first to deduce Conan's true identity.
Ran and Sonoko's classmate, whom Conan finds suspicious. He is later discovered to be looking for his sister, Hidemi and wants to follow in the footsteps of his father and sister to be a CIA agent.
FBI agent Shuichi Akai's civilian persona after he faked his death to protect Kir. He lives in Shinichi Kudo's house, sometimes assists the Detective Boys and Conan on cases, and keeps an eye on Haibara.
Police inspector who works with Inspector Megure. Originally Detective Takagi's rival for Detective Sato's affection, he is currently involved with Sumiko Kobayashi.
Superintendent of the Tokyo Metropolitan Police 1st Division and Former Nagano Police Chief. He has a damaged eye covered by a shaded lens. He knows that Conan is the "Brains behind Sleeping Kogoro".
Inspector from Kyoto Prefectural Police, also known as the "Royal Inspector" for his noble family line. Notorious for his snobbishness and his attachment to his pet chipmunk.
Aka Tooru Amuro. Specializes in detective work and information gathering. He posed as a private detective and became Kogoro Mouri's apprentice. He is later revealed as an undercover National Police Agency agent working to undermine the Organization.
A deceased undercover agent from the Tokyo Metropolitan Police Department's Public Security Bureau. He was killed while infiltrating the Black Organization and the younger brother of Taka'aki Morofushi.
FBI agent, considered by Gin as his rival, and the "Silver Bullet" that could take down the Black Organization. In the past, he infiltrated the Black Organization under the name Dai Moroboshi and obtained the codename "Rye" before being expelled. He faked his death to protect Kir and is currently undercover as Subaru Okiya.
An FBI agent on an undercover assignment against the Black Organization, and Ran's English teacher at one point. She had been Shuichi Akai's girlfriend before he met Akemi Miyano.
Member of the Black Organization. She is an actress and master of disguise. Considered the Boss' "favorite," and knows that Haibara and Conan are really Sherry and Shinichi.. She was known to the world as Sharon Vineyard.
Codenamed Sherry, she was a scientist for the Black Organization and creator of APTX 4869. Her sister's death caused her to betray the Black Organization. Her current alias is Ai Haibara.
Former member of the Black Organization, sister of Shiho Miyano, daughter of Elena Miyano and Atsushi Miyano, and the girlfriend of Shuichi Akai. She was killed by Gin for being a liability to the organization.
An executive member of the Black Organization and a family friend of the Miyanos. His last assignment was to kill a politician. He was killed by Gin for getting caught in the act.
Member of the Black Organization, who pretends to be a patient in the hospital holding Kir. He commits suicide when his cover is blown. His corpse is used to fake Shuichi Akai's death.
Serial killer whom Conan runs into a few times. Former member of the Black Organization, who scheduled to be the original test subject for APTX 4869 before he escaped.
Kaito Kuroba's deceased father. Once a world-renowned magician and the original: Kaitou Kid. He was the one who taught Yukiko Kudo and Sharon Vineyard how to make a perfect disguise.
An anime only character who works for the Magic Kaito Organization as an assassin. His public persona is a stage performer and illusionist named Gunter Von Goldberg II.
An unknown child of middle-school age who appears to be a girl with light hair. She is actually a middle-aged woman and the mother of Shuichi Akai, Shukichi Haneda, and Masumi Sera. Conan suspected that Mary is Asaka, and even the second-in-command Rum in the Black Organization.
A mysterious person from 17 years ago whom Conan believes is the killer of Kohji Haneda and potentially the Black Organization member Rum. This person has been missing ever since.
New deputy homeroom teacher of class 1-B of Teitan Primary school. She is also somehow connected to Kohji Haneda and the case from 17 years ago as she is keeping an object that has the shape of a Shogi piece inside her pocket. She was also seen looking at the updated APTX victim list that contains both Shinichi's and Kouji's names.
Mary's husband and the father of Shuichi Akai, Shukichi Haneda, and Masumi Sera. He went missing 17 years ago afer the murder of Haneda Kouji and his body was never found.
A sushi chief.He has relatively good deductive ability, a "true Tokyoite" personality, and an injury to his left eye, and he was shown looking at Shinichi's name after Shinichi was known to solve the famous Tengu case, making him a suspect of Rum's true identity.