It's unconfirmed intel. We can only hypothesize until we get further intel.
I would say it is doubtful that Takagi and the FBI know. Takagi seems to have lost interest in the matter, and the FBi don't have sufficient information to make the connection between Conan and Shinichi, although Akai may be an exception. Akai has met Shinichi Kudo, and may have made the connection.
Eisuke's sister is a big question mark. Since we haven't seen her for about 60 episodes, I don't have much on HER suspicions.
If Okiya is Akai, then there is reasonable proof to assume that Okiya/Akai knows of Conan's identity.
HOWEVER, if Okiya is Bourbon, then that is a whole different matter. Okiya/Bourbon is likely not fooled by "Kinichi", and will be wondering why Shinichi is hiding. He would also suspect Ran and Suzuki because he may think they are covering him up. Okiya/Bourbon has seen Conan in person, and knows of his detective-like personality, but likely doesn't have sufficient information on Shinichi to make such a connection.