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Forgive me for double posting..!!! >__< But here's a new drawing for Mother's Day!! Px It's Mother's Day~!! Cx Well, enjoy!! x3

[Complete] *all information provided here is from Toxnet unless otherwise noted* Forward note: TTX along with many other natural toxins have very sparse toxicological data on them, though constant research is being done. Structure: Chemical Formula: C11-H17-N3-O8 Appearance: Crystals LOAEL: N/A LD50(GI [assumed]): 0.1mg/kg (from Essentials of Toxicology Second edition by Klassen, C) LD50Perspective: 8.6mg would be necessary to kill an average weight USA male (86kg). However, according to Toxnet, 1-4mg is the common lethal dose for humans. (I know that looks LESS toxic than KCN, but remember KCN was just the CN- ion, let me put the mice values to show you how much more toxic TTX is) LD50(IV mouse): 0.009 mg/kg LD50(IP mouse): 0.008 mg/kg (Btw, this "intraperitoneal" injection, which is basically injection into Body cavity... AKA the space where all of your organs are) LD50(SC mouse): 0.008 mg/kg (This is "subcutaneous," which means under the skin. If you know anyone on insulin, this is the method they usually use to administer their Insulin) LD50(Oral mouse): 0.435 mg/kg Perspective on LD50 of TTX Toxicity: I'm at a loss for calculating the lethality in layman's terms. Basically, TTX is so lethal that a minimal amount can kill you if you don't get treatment (Aka life support). T1/2: N/A. ADME Absorption: IV, IM (intramuscular), GI Tract (Ingestion) Distribution: TTX is distributed by the cardiovascular system (through the blood) Excretion: Urine. Metabolism: Unknown. However it is quickly transferred to the blood and liver upon injection, so it is likely that metabolism at least occurs in the liver, and possibly in the kidneys. However, the exact mechanism of metabolism is still unclear. Mechanism of toxicity (aka: how it kills you): Information on the mechanism from Detective Conan File TTX is as follows: It causes nerve paralysis and respiratory arrest. He also mentioned that death from acute injection occurs faster than ingestion. Verdict: Correct. Technical explanation: TTX is a relatively small molecule that has an affinity for Sodium ion channels. Sodium ion channels are an important part of neuronal signal induction, which allows for the transduction of action potential. Action potential is basically the sharp rise and fall of negatively charged ions in a cell (neuron), and the sodium is what moves the negative ions. Thus, when the sodium ion channel is blocked by TTX, the action potential is blocked, and neuronal activity ceases. This can lead to respiratory arrest, and loss of feeling. Translation for lay people: It stops neurons from working by stopping positive ions from moving the negative ions through the neuron (remember, opposites repel). To simplify it further, neurons have what are known as gates, and TTX locks the gate for Sodium (which is necessary), stopping the neuron from working. Notes on accuracy in DC: Most of what Gosho said was entirely accurate. However, he failed to mention a few things, and also over estimated TTX's lethality SLIGHTLY. 0.5 is not the start of the average. I never found any data that corroborates that value. However, I cannot say that he is entirely wrong, because it is possible for lethality at that dose. What he said about the IV vs GI was also correct, but it lacked perspective. If you look at the numbers, the lethal dosage for IV is MUCH lower than GI. It also has 100% bioavailability from IV injection. Which means that all of TTX can be used, which aids in its toxicity. Also death is not immediate, it is rapid though. Remember that death occurs from respiratory arrest, which is not immediate and would take some time (though not much). Also the other symptoms would also occur (like numbness and muscle paralysis). Overall, there is not much to say, as Gosho was nearly 100% accurate in this case. He just bolstered its abilities as a toxin slightly. Final note: As before, any requests to further clarify will be done to the best of my ability, and anyone who wants to know further symptoms of acute toxicity let me know and it will be added. And for the record, I even consulted one of my professors who is a Toxicologist and has their own lab for Toxicological research on the Metabolism of TTX. She was stumped too. So I didn't leave blanks from lack of trying.

I've decided to allow commenting in thread. In the event that I need more space I'll request a mod split the topic for discussion and reference. However for now, I'm opening the thread for comment and critique. This is especially for the APTX analysis. Welcome one and all to Kyuu's Detective Conan Toxicology thread! I will be posting analysis of the drugs and toxins found in DC that have been used in conjunction with cases (Yes, that includes APTX). There are THREE CATEGORIES of drugs that I'll be doing: 1) Real (Drugs and Toxins that were 100% identified by the series.) 2) Theoretical (APTX basically) 3) Semi-theoretical (Drugs that are possible IRL, but are not identified by Gosho, and can be determined to some degree of accuracy using the information in manga canon) (unnamed poisons and Conan's tranquilizer mostly) Key Terms that you will need to know and understand before reading my analysis: LD50 - Dosage of a toxic agent that will cause death in 50% of treated animals LC50 - Concentration of a toxic agent that will cause death in 50% of the treated animals T1/2 - Biological half-life. How long it takes the concentration in the biological system to be halved. LOAEL - Lowest observable adverse effect level. The amount of a toxin that causes an observable negative effect (your basic symptoms of poisoning without dying, if you have ever seen a drug commercial, you should know most of them) Bioavailability - How much of a drug reaches circulation unchanged in a biological system. IV is always 100% bioavailability, as it is direct to circulatory system. ADME - Absorption, Distribution, Metabolism, Excretion Absorption - How a drug is/can be administered Distribution - how a drug is distributed throughout the body (and what barriers it needs to pass) Metabolism - how the drug is metabolized by the biological system Excretion - how the drug is expelled from the body Those are the main, if I come to a point where I need to explain the more complex terms, I shall append them. I will make every effort to make this easy to read and understand for all. Drugs will be completely analyzed most likely using Toxnet a lot. I'll be using some other resources as well as needed. Everything will be fully referenced. If you use this information for any reason, PLEASE do the same. The drugs currently in planned to be analyzed are: (links will be added as analysis are done) APTX Tetrodotoxin Potassium Cyanide Sea Snake Venom (erabutoxin) Curare (possibly from poison dart case) Batrachotoxin (possibly from the poison dart case) Chlorine Potassium Chloride Phosphoric Acid Carbon Monoxide Aconitine (by Kjeldahl) More may be added as I reread the chapters and watch the episodes that the ones omitted appeared in while I look everything up. If you want clarification please PM or leave comment on my profile. If you wish to critique feel free to do it in topic. Also, this is being done on my own time, so I'm not sure how many I can/will get done. The ones that I am sure of are the top 3, and Carbon Monoxide. Everything else depends on my time and schedule. That all said, I hope you all enjoy this new project of mine and find it illuminating.