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Heads up, James Black, Sonoko, and the Detective Boys (Ayumi, Genta, Mitsuhiko) have all been denied by Gosho via postcard. People Gosho has said are not the the boss in interviews: Agasa, Kudo Yuusaku, Conan, Ran, Vermouth, Shiratori, James Black, Sonoko, Ayumi, Genta, Mitsuhiko

I have some copypasta from here

It is certainly true that James' alibi will be broken if Gin received orders from Rum. The question now is did Gin receive orders from Rum? This is what Gin said to Chianti. Chianti: Are you telling me to quit after coming all the way here!? Gin: Yeah, I just got ano kata's approval. We are changing targets. The alibi is broken if Gin regularly refers to Rum as "ano kata", the same as the boss of the Black Organization. If it turns out there are two ano katas running around then it is going to confuse a lot of issues. Like which ano kata is Vermouth the favorite of?

I feel like there is a reason the boss does not show himself in person to most of his subordinates (except Vermouth), so he would not show up in person to do a loyalty test.

The main reason people think Anokata is male is that a criminal mastermind almost always is male. Probably another reason is the fact that Vermouth is Anokata's favorite, as discussed in another thread (too lazy to dig it up), if Anokata is female the whole thing could get very awkward.